Journal: Scientific Reports

Article Title: The roles of IL-17C in T cell-dependent and -independent inflammatory diseases

doi: 10.1038/s41598-018-34054-x

Figure Lengend Snippet: Generation of Il17c −/− mice. ( a ) IL-17C gene targeting strategy. The region of the Il17c gene containing from exon 1 to exon 3 was replaced with a cassette containing a neomycin resistance gene ( Neo r ), flanked by loxP sequences. ( b ) Expression of Il17c mRNA in various tissues from wild-type (n = 3) and Il17c −/− (n = 3) mice, determined by qPCR. The data show the mean + SEM.

Article Snippet: For IL-17C detection, plates (Nunc) for ELISA were coated with rat anti-mouse IL-17C mAb (MAB2306; R&D Systems; 2 μg/ml in PBS) as a capture Ab at room temperature overnight.

Techniques: Expressing

Journal: Scientific Reports

Article Title: The roles of IL-17C in T cell-dependent and -independent inflammatory diseases

doi: 10.1038/s41598-018-34054-x

Figure Lengend Snippet: Strong resistance of Il17c −/− mice to T cell-independent endotoxin shock. Female mice were injected intraperitoneally with LPS. After LPS injection, survival was monitored, and peritoneal fluids were collected at the indicated points. ( a ) Survival of wild-type (n = 47) and Il17c −/− (n = 46) mice after LPS injection. ***p < 0.001. ( b ) The concentrations of IL-17C, IL-1β, IL-6, IL-17A and TNF in peritoneal lavage fluids from wild-type mice (0 h, n = 5; 3 h, n = 5; 6 h, n = 6) and Il17c −/− mice (0 h, n = 5; 3 h, n = 5; 6 h, n = 6) were measured by ELISA. The data show the mean + SEM. *p < 0.05: wild-type mice vs. Il17c −/− mice. ( c ) Peritoneal F4/80 − and F4/80 + cells were stimulated with LPS for the indicated times. The expression levels of Il17c and Il17re mRNAs in the cells were determined by quantitative PCR. The data show the mean + SEM (n = 3). ( d ) The expression levels of Il17c , Il17re , Il1b , Il6 , Il17a and Tnfa mRNAs in the tissues and peritoneal lavage fluid cells from wild-type mice at 0, 3 and 6 h after LPS injection were determined by quantitative PCR. The data show the mean + SEM (n = 5). *p < 0.05, **p < 0.01 and ***p < 0.001 vs 0 h ( c , d ).

Article Snippet: For IL-17C detection, plates (Nunc) for ELISA were coated with rat anti-mouse IL-17C mAb (MAB2306; R&D Systems; 2 μg/ml in PBS) as a capture Ab at room temperature overnight.

Techniques: Injection, Enzyme-linked Immunosorbent Assay, Expressing, Real-time Polymerase Chain Reaction

Journal: Scientific Reports

Article Title: The roles of IL-17C in T cell-dependent and -independent inflammatory diseases

doi: 10.1038/s41598-018-34054-x

Figure Lengend Snippet: Generation of Il17c −/− mice. ( a ) IL-17C gene targeting strategy. The region of the Il17c gene containing from exon 1 to exon 3 was replaced with a cassette containing a neomycin resistance gene ( Neo r ), flanked by loxP sequences. ( b ) Expression of Il17c mRNA in various tissues from wild-type (n = 3) and Il17c −/− (n = 3) mice, determined by qPCR. The data show the mean + SEM.

Article Snippet: After washing the wells, goat anti-mouse IL-17C polyclonal Ab (AF2306; R&D Systems; 1.6 μg/ml in 1x Assay Diluent), as a detection Ab, was applied to the wells, followed by incubation at room temperature for 1 hour.

Techniques: Expressing

Journal: Scientific Reports

Article Title: The roles of IL-17C in T cell-dependent and -independent inflammatory diseases

doi: 10.1038/s41598-018-34054-x

Figure Lengend Snippet: Strong resistance of Il17c −/− mice to T cell-independent endotoxin shock. Female mice were injected intraperitoneally with LPS. After LPS injection, survival was monitored, and peritoneal fluids were collected at the indicated points. ( a ) Survival of wild-type (n = 47) and Il17c −/− (n = 46) mice after LPS injection. ***p < 0.001. ( b ) The concentrations of IL-17C, IL-1β, IL-6, IL-17A and TNF in peritoneal lavage fluids from wild-type mice (0 h, n = 5; 3 h, n = 5; 6 h, n = 6) and Il17c −/− mice (0 h, n = 5; 3 h, n = 5; 6 h, n = 6) were measured by ELISA. The data show the mean + SEM. *p < 0.05: wild-type mice vs. Il17c −/− mice. ( c ) Peritoneal F4/80 − and F4/80 + cells were stimulated with LPS for the indicated times. The expression levels of Il17c and Il17re mRNAs in the cells were determined by quantitative PCR. The data show the mean + SEM (n = 3). ( d ) The expression levels of Il17c , Il17re , Il1b , Il6 , Il17a and Tnfa mRNAs in the tissues and peritoneal lavage fluid cells from wild-type mice at 0, 3 and 6 h after LPS injection were determined by quantitative PCR. The data show the mean + SEM (n = 5). *p < 0.05, **p < 0.01 and ***p < 0.001 vs 0 h ( c , d ).

Article Snippet: After washing the wells, goat anti-mouse IL-17C polyclonal Ab (AF2306; R&D Systems; 1.6 μg/ml in 1x Assay Diluent), as a detection Ab, was applied to the wells, followed by incubation at room temperature for 1 hour.

Techniques: Injection, Enzyme-linked Immunosorbent Assay, Expressing, Real-time Polymerase Chain Reaction